FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4082830
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG146240.3581829270383533No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG68760.16841259616491502No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC48450.1186676888334807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA51500.035.67890532
GCGGCAC54650.033.6924533
CGGCACC55300.033.05535534
TCGCCGG51050.032.29662748
CCCGTCG51950.031.4112944
CCGTCGC53600.030.97624845
ACCCGCG63150.029.38360625
GCACCCC62950.029.21761536
CCCCCGA63800.029.16771519
AGGACCG8850.029.1082570
TACCGTC854.2900156E-628.5307121
GTTCCGT3650.028.4748781
CACCCCC65150.028.24001937
AGTCCGC67750.027.7149587
CAGTCCG65300.027.5897836
GGCACCC67700.027.26612935
CCCCGAC70050.026.52016320
AGTCGTA5250.026.3465910
GTCCGCC72000.026.1750628
TGGCCGT15300.026.08103870
Produced by FastQC (version 0.11.5)