FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4082830
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG100710.24666714019442398No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC100510.24617728389376975No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC75800.18565553794794296No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT46140.11300984856092465No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC42660.10448634892954152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG21850.060.5891274
TCTACGG22300.059.059365
TACGGCC22250.058.7267
CTACGGC23350.055.8114246
GCGCGGT54900.049.804989
CCGAACT9200.048.18326667
GGTCTAC22950.047.8951763
GCCATAC26950.047.84303711
ACGGCCA27200.047.657478
TGGCGCG58300.046.95854615
CCATACC27900.046.46005612
CGGTTCA9750.045.7355355
CGGCCAT28550.045.5250479
GGGTCTA22500.044.3979262
CGCGGTG62300.043.9446310
CATACCA30000.043.8991813
GAACGCG29850.042.65829524
TGAACGC30900.041.6515523
ATCTCGT30900.041.64993735
CCCGATC30300.041.6287431
Produced by FastQC (version 0.11.5)