FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4024748
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG152310.37843363112423434No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC108200.2688367072919845No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG94710.23531908084680084No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC93350.23193998729858367No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG80650.20038521666449677No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT72630.18045850324045135No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT58790.14607125713212354No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG55140.1370023663593348No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT51230.1272874724082104No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC48530.1205789778639557No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA48260.11990812840953025No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG46210.11481464181111463No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG46130.11461587160239597No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT43960.10922422969090238No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT43010.10686383346236834No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG42970.106764448358009No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG42040.10445374468165461No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG40470.10055287933555095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT28350.050.7944870
GTTCCGT5100.037.966771
ATGGCCG31900.035.5017369
CGCGGCA55900.034.9994432
GCGGCAC57750.034.12617533
CGGCACC58300.033.69339834
AGGACCG7400.033.57496670
GTCGGTT4350.031.7387084
CGTACGT1850.031.73146219
GCACCCC65400.030.6316336
CCCGTCG56250.029.98622144
CGTAGCG6800.029.949439
CCGTCGC56850.029.81001945
TCGCCGG57000.029.59292448
CCCCCGA66600.029.45011519
CACCCCC67500.029.43771237
GGCACCC67350.029.430135
GGTCGGT4700.029.3755513
CGCCCTA11900.029.35119461
TACGGGT15500.028.9505716
Produced by FastQC (version 0.11.5)