FastQCFastQC Report
Wed 8 May 2024
EGAF00005319094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3314232
Total Bases240.7 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN392031.1828683085553455No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG60730.18324003871786887No Hit
GTGGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGG57400.17319246208473033No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50250.15161883658114458No Hit
GTGGGGTGGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGG48790.14721359277202078No Hit
TGGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGG47900.1445282044226234No Hit
GGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGC47560.143502325727348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)