FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005319095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3314232
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN390991.1797303266639148No Hit
GGGCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC142030.42854573849989985No Hit
GGCCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC92890.2802760941298014No Hit
CGGCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC60680.18308917420385779No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC45920.13855396966778427No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG45520.13734705355569557No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC41520.12527789243480844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)