FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005319096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3285913
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN382451.1639078697457905No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG61610.18749735613815705No Hit
GTGGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGG54530.16595083314743878No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50770.15450804692637937No Hit
GTGGGGTGGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGG47600.1448608042878798No Hit
TGGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGG47260.1438260842572521No Hit
GGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGC45800.13938287471396837No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)