FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005319097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3285913
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN381321.1604689472910574No Hit
GGGCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC140940.4289218856372643No Hit
GGCCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC94270.28689134496257207No Hit
CGGCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC61480.18710172789115231No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG45740.13920027706150467No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC45640.1388959476407318No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC41170.12529242253218512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)