FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3314764
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN387751.1697665354154927No Hit
GGGCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC141780.42772275793993175No Hit
GGCCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC96450.2909709409176641No Hit
CGGCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC62460.18842970419613583No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG45810.13819988391330423No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC45510.13729484210640638No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC43100.1300243395909935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG12650.054.6826364
TCTACGG12700.054.4673545
TACGGCC12750.054.2520987
CTACGGC13100.052.799396
GCGTACA3300.047.904251
CGGTATT5250.047.409191
ACGGCCA14750.047.122438
TGGCGCG31950.046.6430815
CGGCATA3050.045.2141271
CGGTATA3850.043.681691
GCGTATA3250.043.466641
GCGCGGT34850.043.4361959
CGGCCAT16250.042.9783029
CCGAACT5150.042.65188667
CGGTACT2550.042.20851
GGTCTAC13750.042.0448723
ATCGCCC69200.042.0063357
TGCGGCG66900.041.9848268
TGGATCG69800.041.51640754
ACGCGAG68500.041.3834633