FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3278906
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN383041.168194513657909No Hit
GGGCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC137100.41812726561847147No Hit
GGCCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC91750.2798189396097357No Hit
CGGCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC58800.1793281051667843No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG45300.13815583612338994No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC45210.1378813543297673No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC41480.1265056088829628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG10350.057.8844154
TACGGCC10400.057.613247
TCTACGG10650.056.2616845
CTACGGC10900.054.6641776
ACGGCCA12950.046.5270358
CGGTATA3300.044.9787941
CGGTATT3700.042.8514181
CGGTACT3000.042.7298551
GCCATAC14000.042.55930711
TGGCGCG31000.042.540615
CGGCCAT14300.041.8998959
GCGCGGT31550.041.5893949
ACGCGAG67650.041.58678433
TGGATCG69650.041.52918254
ATCGCCC69950.041.28312357
GCGTACT2800.040.962831
TGGACGC69250.040.7532430
GACGCGA69300.040.66558532
TGCGGCG68550.040.60191368
GCGTATT3750.040.480911