FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3694910
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC138450.37470466127727065No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC128570.34796517371194424No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA79010.21383470774660276No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG73040.19767734532099562No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT62610.16944932352885456No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC45700.12368366211896907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC17450.057.0006877
GTCTACG17400.056.9683884
TCTACGG17550.056.2839935
CTACGGC18850.052.5854956
GCGCGGT69000.051.531689
CCGAACT10000.048.26920767
CGGCCAT20500.048.1800239
TGGCGCG75850.047.1069515
CGCGGTG76000.046.9221410
ACGGCCA21400.046.6395878
GCCATAC21550.046.15413311
CGGTTCA10750.046.1046525
CGAACTT11100.044.57815668
GCCGGTT11150.043.8289873
CGGTCTA4100.043.125962
CATACCA23050.042.85049413
GGGTCTA17950.042.4923442
GAACGCG23400.042.38981224
CCATACC23650.042.34895712
CGTGCCT83800.042.1944820