FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3697717
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG182490.4935207318461634No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC101190.27365533922687973No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC76580.2071007597390498No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG72520.19612101196495027No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG69080.18681797444206788No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA56280.15220202086855214No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG47360.12807902822200834No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT43050.11642318760467607No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC39640.10720128122298164No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT38480.10406421043038178No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA37960.1026579373164577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA52650.037.13099332
GTTCCGT4800.035.52851
GCGGCAC55850.035.1316833
CGTACGT1201.8189894E-1234.6833319
CGGCACC58850.033.34528734
TCGCCGG54050.033.3051648
CCCGTCG54500.032.74747544
ACCCGCG62050.032.09547425
CCGTCGC56000.032.0601745
CCCCCGA63800.031.80211619
CACCCCC65200.030.69656837
GCACCCC64400.030.642136
GTCCGTT2750.029.0063082
CCCCGAC69950.028.85927820
GGCACCC69450.028.26084735
CCCGCGC73700.027.16547426
CCGCGCG73800.026.94424227
AGTCCGC74950.026.8345897
TTCCTCG9050.026.438644
CAGTCCG73200.026.4339286
Produced by FastQC (version 0.11.5)