FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3697717
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC139230.3765296262531719No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC125580.33961495701266486No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA76790.2076686777273653No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG74930.2026385469737138No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT63850.1726741121616392No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC45740.12369794659786025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG17850.060.0123565
GTCTACG17800.059.7914164
TACGGCC18600.057.778897
CTACGGC19650.054.690736
CCGAACT11000.053.4587867
GCGCGGT72400.051.857359
CGAACTT11800.050.69203668
GCCGGTT11250.050.5386353
ACGGCCA21850.049.1847768
TGGCGCG77350.048.31334715
GCCATAC22350.047.92868811
CGCGGTG78800.047.60159310
GCGTCTA4750.047.442082
CGGCCAT23100.046.9734889
CGGTTCA12500.046.59295
CATACCA24050.044.3966513
CCATACC24300.044.3678812
GCCCGAT23900.044.1434730
ATCTCGT23900.044.02688635
CGTGCCT87050.043.3813420
Produced by FastQC (version 0.11.5)