FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3704420
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG181520.4900092322144898No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC102340.2762645704320784No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC78750.21258388627639416No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG75540.20391856215008017No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG70010.1889904492471156No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA58350.1575145366886044No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG47410.12798224823319168No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT41900.11310812488864654No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC40290.1087619654358847No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA38880.10495570156731687No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT38410.10368694694446094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA53450.036.44037632
GCGGCAC55100.035.4799733
GTTCCGT4250.034.5526471
CGGCACC56600.034.5443634
TCGCCGG52350.033.25494448
CCCGTCG53300.032.89719444
CCCCCGA62100.032.16314719
ACCCGCG62150.032.03910425
CCGTCGC54700.031.93450545
GCACCCC62900.031.5363736
TGGCCGT13200.031.20599770
TTCCTCG8950.030.2334024
CCCCGAC66850.029.82714520
CACCCCC66550.029.75975837
GGCACCC67300.029.31295435
GTCGGTT2900.028.7097024
ATGGCCG11050.028.37769369
AGGACCG4500.027.907870
AACGCGT500.007531546527.752712
AGTCCGC74250.027.0347187