FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3704420
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC132590.35792377754142346No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC120740.325934964177928No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA70910.1914199793759887No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG70570.19050215688285888No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT61410.16577493912677288No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC43070.11626651405618155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG16950.057.4771275
GTCTACG17150.057.2049754
TACGGCC17350.056.351077
CTACGGC18350.053.2808886
GCGCGGT72250.053.264489
CCGAACT11400.050.62845667
CGCGGTG78050.049.35074210
TGGCGCG78850.048.80145315
CGAACTT12300.047.97195468
GCCGGTT12350.047.157833
CGGTTCA12300.047.0727735
ACGGCCA20950.046.8333248
CGTGCCT87850.043.61420
CGGCCAT22800.042.729139
GTAGTCC90250.042.59188527
CTACTCG89950.042.5658337
TACTCGG90400.042.3969738
GAACGCG22550.042.29922524
GCGTCTA4850.042.1894342
GCGTGCC91200.042.16345619