FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3569983
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA246040.6891909569317277No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC132290.37056198867053425No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC89340.25025329252268147No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT75310.21095338549231185No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC75040.21019707936984575No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA64110.17958068707890207No Hit
GGGGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTC57830.16198956689709726No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA55140.15445451701030508No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG51440.1440903219987322No Hit
GGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTC48880.13691941950423855No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT47350.1326336848102638No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA41550.11638710884617658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGACT48800.051.570361
TCTACGG4650.050.651125
GCGCGGT52200.050.2958079
ACTCTTC60300.049.8006325
GGACTCT60500.048.544843
TCTGGTC60750.048.519510
TTCTGGT61500.048.0967649
CGCGGTG55800.047.11298410
TGGCGCG56400.046.91489815
GGGACTC59300.046.6069642
GTCTACG5150.045.733534
CGGTATA1450.045.6107561
CTGGTCC64800.045.4259411
CGGGACT11750.045.324731
CCGAACT8500.045.19616367
CGGTTCA8600.045.10795
TCTTCTG66850.044.972957
CTCTTCT67600.044.781416
GCCGGTT8650.044.4436263
GACTCTT69800.044.2135474
Produced by FastQC (version 0.11.5)