FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3550390
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA244350.6882342503217956No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC130640.36795957627190257No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC89330.25160616157661553No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC76970.2167930847033706No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT76010.21408915640253606No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA61680.17372739332862022No Hit
GGGGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTC57370.1615878818946651No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA54660.15395491762876756No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG52840.14882872022510202No Hit
GGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTC49320.13891431645537533No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT48140.13559073791893286No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA40410.11381848191325461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT7800.053.27576467
GGGGACT51050.052.868541
GCGCGGT49400.051.90469
ACTCTTC62350.051.407295
GGACTCT61200.051.0710263
TCTGGTC64100.049.9476510
GGGACTC60150.049.4993442
TTCTGGT65550.048.895649
CGAACTT8850.048.43628768
TGGCGCG53400.047.75706515
CGGTTCA9150.047.7165345
CGCGGTG54150.047.47954610
CTGGTCC68250.046.80911
TCTTCTG69350.046.6161357
TACGGCC4800.046.1997687
GACTCTT71300.046.1207244
CTCTTCT70150.046.0370676
GCCGGTT9350.045.9546553
CGGCATA1600.045.7668951
GCCGAAC9350.045.064966
Produced by FastQC (version 0.11.5)