FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3344436
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN314170.9393811094008078No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG184710.5522904310323176No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG73500.21976799675640377No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA58410.17464828150396658No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG55470.16585756163371043No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG47400.14172793260208896No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC45390.13571795064997505No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC45000.13455183474881866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT18200.039.5049170
CGCGGCA52650.037.64095332
GCGGCAC54700.036.2204333
CCGTCGC52200.035.37957445
AGGACCG8400.035.3540370
CCCGTCG51800.035.32932744
TTCGTTG3900.035.29251531
TCGCCGG52500.035.0953248
CGGCACC56350.034.96080834
TAGTCCG3300.032.66766
GCACCCC61600.032.36300736
CCCCCGA60100.032.3585919
ACCCGCG61050.032.26682325
CACCCCC64100.030.83424837
TATCGGA2300.030.7590318
GGCACCC65200.030.3219235
CCCCGAC65150.030.14512420
ATGGCCG19850.029.5025569
CCGCGCG70200.028.33320827
TTATCGG2600.027.2099117