FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3328087
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN312520.939037951832389No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG183980.5528100677656564No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG69700.20942962128093406No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA56960.17114937199658542No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG54040.16237556289844587No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG47040.14134245889605648No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC44640.13413110895238015No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC43070.12941368419755853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT18200.045.20339270
CGCGGCA56750.036.56469332
GCGGCAC58100.035.95007733
CGGCACC59150.035.02279334
TCGCCGG57300.034.3545848
CCGTCGC58050.033.72384345
CCCGTCG59050.033.33724244
TTCGTTG5000.033.02420831
GATTTCG3250.032.6473728
ACCCGCG64650.031.71642925
ATGGCCG20000.031.47864569
GCACCCC66650.031.39262836
CCCCCGA65050.031.31588219
CGTTGGA5100.031.13925633
GTTCCGT2900.030.4121551
CACCCCC68650.030.32666837
GGCACCC69450.029.9726435
ATTTCGT3600.029.52299929
TATCGGA2400.029.4334058
CCCCGAC70050.029.21358720