FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3328087
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN310100.9317665073058488No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG139580.4194000938076438No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC82400.24758968139955476No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA69610.2091591956580462No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC66590.20008491364558678No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG38320.11514122076736577No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT35910.10789982353225742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG30700.062.652715
GTCTACG30850.062.239084
TACGGCC31250.061.9766777
CTACGGC31950.060.201516
ACGGCCA35300.055.150138
GCCATAC35450.054.72707711
CGGCCAT35900.054.2283979
CCATACC37450.051.62405412
GGCCATA38400.050.87302810
CCTGGTT53750.050.07147270
CATACCA39100.049.44554513
GGGTCTA28450.049.4191062
GCGCGGT41400.049.054559
CCGAACT6950.048.74077667
GAACGCG38700.048.22891224
TACCACC40350.047.99710515
GCGTCTA7350.047.1357842
CGTCTAC8250.046.8733633
CTGAACG40250.046.32464622
TGAACGC40400.046.2155723