FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3333990
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN314580.9435541198383919No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG186450.5592398297535386No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG73240.21967672368543395No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA59390.17813490742323762No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG55010.1649974954933878No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG46720.14013239391839807No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC44380.13311377658601256No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC43500.13047429656357698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT17400.041.00368570
CGCGGCA54200.036.62209732
GCGGCAC56350.035.27730633
CGGCACC57700.034.44077334
TCGCCGG54900.034.28241348
CCGTCGC55550.033.95498745
CCCGTCG56300.033.46292544
TTCGTTG4550.032.51654431
TAGTCCG3450.032.186716
ACCCGCG62600.031.7900625
GCACCCC65200.030.89650236
CACCCCC65250.030.6127337
CGACGTT553.769926E-430.592993
CCCCCGA65100.030.48699419
ACGATCG902.4852488E-729.9108836
GGCACCC67600.029.59816235
ATGGCCG19050.029.50779369
AGGACCG8550.029.23658870
CGTTGGA5200.029.2022433
TTATCGG2200.029.061147