FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3333990
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN311850.9353657329506087No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG141720.42507625997678455No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC83090.24922090348201403No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC67610.20279005036007908No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA67400.20216017444563422No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG38650.11592716234901723No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT36670.10998833229853718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG29800.061.2870065
TACGGCC30150.060.463997
GTCTACG30250.060.2641144
CTACGGC30600.059.5721136
ACGGCCA32950.055.4288
CGGCCAT33100.055.1768239
GCCATAC34800.052.38554411
CATACCA35950.050.9904713
CCATACC35850.050.9450612
GCGCGGT45250.050.9162989
TGGCGCG45800.050.30333315
GGGTCTA28750.049.606562
CCTGGTT52750.049.0669570
GGCCATA37650.048.5980410
CTGAACG37800.047.30065522
GAACGCG37900.046.75869424
CCGAACT6400.046.60315767
TACCACC39400.046.52345315
TGAACGC38750.046.1027523
CGGTATA2350.046.100931