FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3309245
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN308480.932176372556278No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG136980.4139312743541201No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC80090.24201894994175407No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA65070.1966309535860899No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC63890.19306518556347446No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG35710.10790981024372628No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT34780.10509950154793615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG28050.062.1589285
GTCTACG28300.061.7155724
TACGGCC28350.061.619897
CTACGGC28600.061.3166356
CGGTATA2350.057.7331961
ACGGCCA31800.055.146418
GCCATAC32500.053.440811
CGGCCAT32750.053.3411879
CCATACC35500.049.38971312
CGTCTAC8500.049.0926633
GGCCATA35750.048.9591610
CATACCA35900.048.83940513
GGGTCTA24850.048.762142
CCTGGTT51150.048.10239870
GCGCGGT42500.047.8359769
TACCACC36500.047.57411215
GCGTCTA7150.046.605352
GCGTACT2200.046.2521551
TGAACGC36800.046.04126723
TGGCGCG44500.045.8275315