FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3179057
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG173690.5463569857350781No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG61380.19307612288801365No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55320.17401386637609834No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG45750.14391059990431126No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA37690.11855716962608724No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG37520.118022419855951No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC37010.11641817054554228No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC36220.11393315690785034No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC33940.10676121881425843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA51350.038.310832
AGGACCG6500.038.25589470
GCGGCAC52350.037.5950633
CGGCACC54650.036.03453434
TAGTCCG2450.035.1495826
ACGATCG400.002616826734.446596
TGGCCGT14350.034.3032770
TCGCCGG53300.033.8720348
CCGTCGC53000.033.59920545
CCCGTCG53450.033.36451344
GCACCCC59850.033.1058536
TTCGTTG4750.032.7994931
CCCCCGA60750.032.62638519
ACCCGCG62200.031.53604325
GGCACCC63300.031.17652535
CACCCCC63350.031.12679537
CCCCGAC63750.031.04772620
CGTTGGA5150.030.9492233
CCCCCGT59300.029.98137942
GTTCCGT4850.029.9394721