FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3162341
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC129230.4086529567810682No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC123940.39192484301977554No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG111040.35113227827106563No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT61430.19425482577622083No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54010.17079119550990862No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA52670.16655382831895738No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC37580.11883601420593162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG21700.061.0401464
TCTACGG22100.059.935355
TACGGCC22300.059.3940477
CTACGGC23000.057.4395566
CGGCCAT25550.052.242149
GCGCGGT63450.052.158329
ACGGCCA26050.051.1073538
GCCATAC25900.050.87204411
CCATACC26400.049.77824812
CCGAACT10400.049.74554467
TGGCGCG66750.049.5281715
CGCGGTG67250.049.41570710
GCCGGTT10250.047.662743
CATACCA27750.047.48057613
CGGCGTA950.047.3633771
CGAACTT11350.046.79643668
GAACGCG28150.046.31075724
CGGTTCA10800.046.1911545
GGGTCTA22250.046.0926362
GGCCATA29650.045.25015310
Produced by FastQC (version 0.11.5)