FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3170427
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG174960.551849955857681No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG61760.19480025876640591No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54030.17041868492792928No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG45680.14408153854354636No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA38600.1217501617290037No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG37140.11714510379832116No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC36600.11544186319382216No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC35200.11102605421919509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA51850.040.15207732
TGGCCGT15400.039.51161270
GCGGCAC53250.039.11757333
CGGCACC54300.038.43476534
TCGCCGG52400.037.5359248
CCGTCGC53250.036.87056445
CCCGTCG53550.036.39303644
CGAAACG806.705886E-834.72534637
CCCCCGA60350.034.65727619
GCACCCC61350.034.2811736
ACCCGCG62050.033.55991425
CCCCCGT58650.033.36512442
CACCCCC64100.033.0459637
ATGGCCG14450.033.00081669
CCCCGAC63250.032.96867420
GGCACCC64650.032.5142335
GTTCCGT5000.031.1026631
CCGCGCG68450.030.48994327
AGGACCG5500.029.50270
TCGCCGT3750.029.4899331