FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3153350
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC126540.4012875196219893No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC121960.3867632834921591No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG110200.3494696116828135No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT60340.19135205416461856No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN53760.1704853568427228No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA49120.1557708468771307No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC34320.10883663405584537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG24800.061.0459864
TACGGCC24700.060.881037
TCTACGG24950.060.6857225
CTACGGC25150.060.2031366
ACGGCCA27700.054.784338
GCCATAC27600.054.35857411
CGGCCAT28950.052.2991569
GCGCGGT66000.052.2934889
CCATACC29650.050.7130512
CGCGGTG69000.050.21933710
TGGCGCG68400.050.15279815
CCGAACT10000.049.9522667
CGGTATA1900.049.2805331
TGAACGC30550.048.8617223
CTGAACG30750.048.75338722
CATACCA30650.048.60941713
GAACGCG30400.048.55281424
CCTGGTT43250.048.025170
GGCCATA31400.047.99931710
GCGTCTA6600.047.4530372
Produced by FastQC (version 0.11.5)