FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3988146
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC138750.34790601948875494No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC117850.29550071637297126No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA86540.21699305893013945No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG83970.21054896184843785No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT65210.1635095605827871No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC47080.11804984070292311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC18400.058.3114937
GTCTACG19250.056.274684
CTACGGC19300.055.9495626
TCTACGG19250.055.9150965
GCGCGGT65750.052.58729
TGGCGCG69650.049.2413715
CCGAACT11200.048.90270267
GCCGGTT11550.048.8402373
CGCGGTG70900.048.71858210
GCCATAC22200.048.16769811
ACGGCCA22800.047.21028
CGGCCAT23550.045.706699
GAACGCG23350.045.38667324
CGGTTCA12350.045.118885
GCCGAAC12300.044.6461266
CGAACTT12800.043.8073568
TGAACGC24550.043.72862223
CTGAACG24650.043.40583822
CCATACC24950.043.2790812
CCGGTTC12950.043.028434