FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3968843
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC139010.35025320981454794No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC117450.29593007332363613No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA85120.21447056484723634No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG82120.2069116868568497No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT64310.16203714785392115No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC45540.11474376789406887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG17800.059.5102584
TCTACGG18100.059.0976645
TACGGCC18200.058.959447
CTACGGC19500.055.2091036
GCGCGGT65200.052.077679
CGGTATA2250.051.0737341
CCGAACT10750.050.59248467
ACGGCCA21800.049.3780638
TGGCGCG68500.049.316815
GCCATAC22200.048.80019411
CGCGGTG70750.048.139210
CGGTTCA11500.047.5608525
GAACGCG22550.047.01573624
CGGCCAT22750.047.011849
GCCGGTT12050.044.8154683
CCATACC24500.044.3602212
CCCGATC23800.044.15204231
GCCCGAT23900.044.107430
ATCTCGT24150.043.55066335
CGAACTT12900.043.50767568
Produced by FastQC (version 0.11.5)