FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3974554
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG162070.4077690226375085No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC79750.20065144416203679No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG74990.18867525765155033No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC64460.16218171900545317No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG61940.15584138497048977No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA52180.13128517061285366No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC47050.11837806204167813No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG41050.10328202862509857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA49000.036.95514732
GCGGCAC50400.035.86974333
CGGCACC50900.035.18052734
TCGCCGG48250.034.9501348
CCGTCGC49250.034.2725745
CCCGTCG49300.034.0953344
TAGTCCG3100.033.495446
CCCCCGA57150.031.98550619
ACCCGCG57400.031.56529425
GCACCCC57950.031.08808736
CCCCGAC60950.029.88224420
CACCCCC61000.029.65498237
GGCACCC61300.029.38543735
GTTCCGT5000.028.481491
GATAGGC26500.027.8054440
AGTCCGC67800.027.1586467
TGGCCGT12050.026.81555770
ACGTATC3000.026.68196145
ACCGGTT1950.026.6252523
GTAGCGT3400.026.46796810