FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3979184
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG161900.4068673376250005No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC78610.19755306615627724No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG72350.18182119751185166No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC66820.167923875849923No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG63200.15882653327918486No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA49760.12505076417677594No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC48160.12102983928363202No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG41160.10343829287612738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA50150.034.9355632
GCGGCAC51650.033.9239633
AGGACCG7000.033.06648670
CGGCACC53550.032.8552834
TCGCCGG50450.032.81248
TGGCCGT15050.032.43123670
CCGTCGC50450.032.2945245
CCCGTCG51400.032.02818344
GTTCCGT4800.031.3044261
GCACCCC58800.030.1159136
ACCCGCG60050.029.54428725
CCCCCGA60900.029.39902519
CGTACGT1452.1827873E-1128.65978419
CACCCCC63300.028.14769437
GGCACCC63650.027.8658535
TAGTCCG4100.027.8621716
TTACCCG4600.027.8438179
GATAGGC26300.027.75409940
CGCGCTT4750.027.69261214
ATGGCCG14050.027.30512869