FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5081743
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC312670.6152810167692463No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC293570.577695487552204No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG237980.4683038870718177No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN163100.32095286991097344No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT157330.309598497995668No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA119150.23446679613667987No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC84770.16681284354600381No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG78030.15354967773852396No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG68930.13564243606967138No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC68830.13544565319418947No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC66080.13003412411843732No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC64720.12735787701188353No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT61610.12123792958439654No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC60270.1186010390529391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG49950.061.763084
TCTACGG50100.061.715615
TACGGCC50100.061.529797
CTACGGC51900.059.516196
CGGTTCA22550.056.0926675
ACGGCCA55650.055.5782438
CGGCCAT55900.055.268359
GCCATAC55700.055.22054711
GGGTCTA41550.054.7828642
GCCGGTT22850.054.60563
GGCCATA57200.054.07219310
GCGCGGT157650.054.028279
CCGAACT23700.053.8438367
CATACCA57600.053.27839713
CCATACC57950.053.07599612
CGCGGTG165550.051.42934810
CGGTCTA6150.050.7240562
TGGCGCG169800.050.4078915
CCTGGTT83200.050.30146470
CCGGTTC25350.049.896534