FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5011356
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC308470.6155419810526332No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC289310.5773088162166089No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG232420.4637866477655948No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN159980.319234953573444No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT157580.31444583062947434No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA118050.23556498480650748No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC83520.16666147845014403No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG75360.1503784604406472No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG67090.1338759409628851No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC66060.1318206090327648No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC65450.13060337361783916No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC64720.12914668205571506No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT59840.11940879873631009No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC58520.11677478111712677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG51200.063.3328484
TCTACGG51700.062.6584365
TACGGCC53200.061.085067
CTACGGC53900.060.2917446
GGGTCTA41150.057.059582
GCCATAC57350.055.82795711
ACGGCCA57950.055.664018
CGGCCAT58350.055.0474249
CGGTTCA23900.054.6468735
CCGAACT24350.054.64105667
GCGCGGT155000.054.3832449
GCCGGTT24000.053.8440783
CCATACC59600.053.83323712
GGCCATA60650.052.95988510
CATACCA60600.052.83176813
GCGTCTA11850.052.6454542
CGCGGTG162900.051.76691410
CGAACTT26400.051.44331468
TGGCGCG165850.050.6389315
CCTGGTT86050.050.27955670