FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3458552
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG327710.9475352690952745No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA252940.7313465288363453No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC246090.7115405522311071No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC213030.6159514155056799No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT102600.2966559415616709No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG88660.25635005632414953No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG60810.1758250273524874No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA50600.14630400236862132No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA50210.14517636282467344No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC50150.14500287981791224No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC43860.1268160779424453No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC42690.12343315931060166No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA39380.11386268010427486No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC36160.10455242540808986No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG36160.10455242540808986No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC35980.10403197638780622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG62300.063.7751054
TCTACGG62550.063.465985
CCTGGTT91300.062.91803470
TACGGCC63800.062.224337
CTACGGC64350.061.7996986
GGGTCTA49300.059.674512
CGGCCAT67000.059.355389
ACGGCCA67100.059.266928
GCCATAC66750.058.9576211
GGCCATA69600.057.23719410
CCATACC68900.057.11704312
CATACCA70200.056.1559613
GCGCGGT109000.055.8500829
CGGTCTA7050.055.7702372
GCGTCTA11450.055.1229322
CCGAACT14250.054.7184167
CGCGGTG111800.054.26622410
TACCACC72900.054.21803715
ATACCAC73000.054.14377214
CTGAACG74350.053.07449322
Produced by FastQC (version 0.11.5)