FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3640542
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG174360.4789396743671684No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG76890.21120481510720107No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG49890.13704003414876137No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC43020.11816921766044726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA52000.037.2112532
TATCGGA2000.036.30648
GCGGCAC54050.035.93416633
TCGCCGG52600.034.59803448
CCCGTCG54000.034.03134544
CCGTCGC54550.033.63791745
CGGCACC57550.033.6347334
CACCCCC63550.030.4839537
GCACCCC63850.030.43980636
GTTCCGT3200.030.352551
TTATCGG2550.029.8315897
CCCCCGA66850.029.17757419
GGCACCC68250.028.62599635
ACCCGCG68550.028.57807225
CGTAGCG4650.028.2553469
AGGAGGG94150.027.8415170
CGTCGCC67050.027.48289946
GTTATCG2650.027.4010586
GGCGATC11100.027.04021570
CGCCCTA13150.026.99590961