FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3639735
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG188040.5166310184670038No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG80180.22029076292642186No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG54440.1495713286818958No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC42490.11673926810605718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA58750.033.69376432
GCGGCAC60450.032.75343733
TCGCCGG59050.031.7700248
CCCGTCG60000.031.37565444
CGGCACC63600.031.19519834
CCGTCGC60500.031.01977545
GCACCCC68550.028.95856936
CACCCCC70800.028.04601537
ACCCGCG72450.027.61700625
CCCCCGA72850.027.58248319
AGGAGGG99000.026.9530470
GGCACCC76200.026.36429835
AGTCCGC79300.025.8109477
CGCCCTA13800.025.47886361
CAGTCCG78250.025.4047556
CCCCGAC79450.025.29361220
TCCGCCC84350.025.2903869
CGTCGCC74600.025.26377746
GTCCGCC82500.024.8517048
TCGTTTG16650.024.71257417
Produced by FastQC (version 0.11.5)