FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3639735
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC71520.19649782195681828No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC65720.1805625959032732No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC49700.13654840256227446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT37400.046.941869
TGGCGCG40100.043.86616515
CGGTATA1300.040.0045621
GTCTACG4250.039.8451354
CGGTATG2650.039.2497631
CGCGGTG45300.038.98172410
TACTCGG47150.038.20557838
CTACTCG47000.038.17085337
CGGCATT5650.036.818361
CGGCATA2750.036.561751
TAGTCCC49600.036.0867728
GTAGTCC50050.035.82161727
TCTACGG4450.035.7244875
GGGGTAG18250.035.715491
CCGAACT6400.035.50960567
ACTCGGG53000.034.91744239
TGTAGTC51800.034.7402826
GCCGGTT6700.034.559563
CGGTTCA6450.034.2916565
AGGATCG51200.033.79042459
Produced by FastQC (version 0.11.5)