FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3741702
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG191740.5124405952157601No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG82540.22059479883753436No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG54800.1464574142996957No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC44680.11941089910420447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA60450.036.3707832
GCGGCAC62950.034.93618433
TCGCCGG60550.034.3142948
CGGCACC64300.034.20911434
CCCGTCG60850.034.08216544
CCGTCGC62950.033.07354745
TACGCGT652.2663718E-531.9611551
CACCCCC73500.030.28196137
GCACCCC73200.030.20294236
CCCCCGA74000.029.92114619
ACCCGCG76050.029.23491325
GGCGATC11400.028.50990370
CGCGCTA1103.5142875E-828.35198228
GGCACCC77900.028.33080735
AGGAGGG105250.028.07712270
CCCCGAC79950.027.65516720
CGTCGCC75900.027.34986946
TAGTCCG3300.027.2409486
AGTCCGC83050.027.227077
CAGTCCG81550.026.6679426
Produced by FastQC (version 0.11.5)