FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3741702
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC72950.19496475133508762No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC65880.17606960682598455No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC50860.13592744692121395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT38200.047.1411829
CGGCATA3450.046.2401541
TGGCGCG39650.044.53835315
CCGAACT6250.041.99841367
GCCGGTT6800.040.148993
GTCTACG4150.038.3059464
CGCGGTG47550.037.9442610
CGGGTAG5950.037.3029821
GGGGTAG19350.037.099661
CTACTCG49100.037.0422737
TACTCGG49250.036.9428538
CGGTTCA7250.036.7036445
GTAGTCC49550.036.61342627
TAGTCCC49950.036.5394228
TCTACGG4400.036.129475
CGGTAAG2500.036.0673181
CGTGCCT50450.035.6499420
TGTAGTC52000.035.3487526
CGAACTT7650.034.9988268
CGGGAAT6650.034.940871
Produced by FastQC (version 0.11.5)