FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3700466
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG187250.5060173502472392No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG79010.21351364936199926No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG54210.14649506305422075No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC42210.11406671484077952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA56850.036.4617132
GCGGCAC59650.034.81678833
TCGCCGG57050.034.09395248
CCGTCGC58150.033.7018745
CCCGTCG58350.033.5758444
CGGCACC61800.033.2765834
CACCCCC68750.030.34332337
TAGTCCG3450.030.0663496
GCACCCC70550.029.45835536
CCCCCGA71850.028.83185419
ACCCGCG72700.028.520625
GGCACCC74250.028.02989635
GTTCCGT2750.027.7014981
AGGACCG8000.027.56092370
CGTCGCC71950.027.25158146
AGGAGGG100300.027.17872270
AGTCCGC78350.026.6107357
CAGTCCG76500.025.9435236
CCCCGAC79900.025.88653220
TCCGCCC83750.025.5142149
Produced by FastQC (version 0.11.5)