FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3700466
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC71000.19186772692952728No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC62890.16995156826194324No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC49400.13349670014533305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT37250.047.7747889
TGGCGCG41000.043.5725315
CGGCATA2700.039.83651
CCGAACT6250.039.77636767
CGCGGTG45600.039.10233310
CTACTCG48300.037.7133837
TACTCGG48250.037.6897638
CGGCAAT4650.036.56171
GCCGGTT6550.036.401543
GCGCATA2100.036.348511
GTCGTAC1350.035.83493
GTCTACG4200.035.3778274
CGAACTT7650.035.0342168
GTACGCA3550.034.77757365
ACTCGGG53500.034.71893339
TAGTCCC52850.034.64962428
GTAGTCC52950.034.446927
CGGATTG3650.034.2210161
TGCGACA7300.034.1163324
CGTGCCT53500.033.9894120
Produced by FastQC (version 0.11.5)