FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3375479
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC103570.30683052686744605No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG94720.2806120257302741No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG77000.22811577260590274No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC75050.22233881472822078No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG46110.1366028347384179No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG44590.13209977013632732No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT44430.13162576333610726No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT39620.11737593390449177No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT38960.11542065585358405No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG33990.10069681962174849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT22500.048.5044770
ATGGCCG24650.034.08402669
CGCGGCA29450.033.7326832
CTTACCC16050.032.87055270
CGTACGT1400.032.04450619
GCGGCAC31800.031.5767433
CGGCACC31700.031.2496734
TCGCCGG31250.030.22063648
CCGTCGC31250.029.94468745
CCCGTCG32800.028.83399844
GATAGGC34250.028.37032940
AGGACCG6200.028.36410570
GTTCCGT3450.028.0851631
TTATCGG2000.027.5973427
ACCCGCG36350.027.27675425
TATGGCC28900.027.23439468
TCGGATA5000.027.01675634
CCCCCGA37000.026.95469319
CGGATAG5100.026.49214635
ATAGGCA36600.026.46615841