FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3356924
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG251010.7477381078630317No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC163520.48711260666014483No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA148630.4427565235316617No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC146800.43730510431573666No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT70520.2100732694573961No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG70490.20998390192926622No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC56580.1685471580530271No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG46890.1396814464670633No Hit
GGCCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC38100.11349676072499705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG48900.064.0050664
TCTACGG49800.062.986935
TACGGCC50200.062.41637
CTACGGC52000.060.3220946
ACGGCCA53350.058.7956738
GGGTCTA40550.057.9594082
CGGCCAT54900.057.1985449
GCCATAC54500.056.9167711
CCTGGTT79450.056.66008870
CCATACC56750.054.78175712
GGCCATA57450.054.47463210
GCGTCTA9100.054.2328382
GCGCGGT75450.053.9682929
CATACCA57700.053.70040513
CCGAACT11900.051.5041967
CGCGGTG78850.051.4615310
CGTCTAC10600.051.4359933
TGGCGCG80300.050.363415
TACCACC60850.050.2430615
CTGAACG62200.049.7377422