FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3446388
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC101930.29575892209466836No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG101340.29404698484326197No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG80460.23346181567484567No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC78450.22762962266581707No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG48150.13971148924613247No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT47400.13753529782485316No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG46830.13588139234468086No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT41270.11974855994159683No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT39020.11321998567775884No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG35910.10419604525085394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT22750.044.61072570
CGCGGCA31150.036.2223132
GCGGCAC32800.034.83035333
CGGCACC34350.033.1665934
TCGCCGG33050.032.3579948
CTTACCC15900.031.58924370
CCGTCGC33850.031.33187145
ATGGCCG25300.031.16057469
CCCGTCG34300.031.00313644
ACCCGCG39050.029.11531825
AGTCCGC39300.028.7137437
CAGTCCG38600.028.519246
CCCCCGA39250.028.4989719
GTTCCGT4450.027.9577241
CCCCCGT38200.027.90194342
GCACCCC41850.027.6586436
GATAGGC32550.027.39564540
CCCCGAC41700.027.07845120
CATTAGC32950.027.02084213
GCTAACC7550.026.968225