FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3725210
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG201880.5419291798314726No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC99470.2670185036548275No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC80650.21649786186550557No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG77640.20841778047412093No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG61200.16428604025007987No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG58110.15599120586490425No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT55310.14847485108222086No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT47420.12729483706958802No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46020.12353665967824631No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA44060.11827521133036796No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT38460.10324250176500116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT16400.041.25183570
CGCGGCA63400.037.10256232
GCGGCAC65600.035.87375333
CGGCACC67500.034.8253234
TCGCCGG64200.033.9087948
CCCGTCG65650.033.1508544
CCGTCGC65500.033.07983845
ACCCGCG73700.032.0400325
CCCCCGA74350.031.90898519
GCACCCC74750.031.88744436
CACCCCC76650.030.79347637
CCCCGAC77300.030.74418420
GGCACCC76650.030.67844435
ATGGCCG17250.030.58727669
AGTCCGC81100.029.6141137
CAGTCCG79100.029.1850196
CCGCGCG81050.029.10319327
GTCCGCC83750.028.7182738
TACGGGT14950.028.6213956
GATAGGC31350.028.49074240