FastQCFastQC Report
Wed 8 May 2024
EGAF00005319183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3725210
Total Bases279.2 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT337670.9064455426673933No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG160130.42985496119681843No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG128630.34529596989163025No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA83700.22468531975378567No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT76910.20645815940577844No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT66020.17722490812598485No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46000.12348297142979858No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG44710.12002007940491945No Hit
GCGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT43570.11695984924339836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)