FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3725210
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC162290.4356532920291742No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG158300.4249424864638504No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC146990.3945817819666542No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA121330.3256997592082057No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT79240.21271284034994No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46000.12348297142979858No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG44200.11865102906950212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG31600.063.3166284
TCTACGG31750.062.8001985
TACGGCC32650.060.8586127
CTACGGC34000.058.64436
ACGGCCA35950.055.464068
GCGCGGT80850.054.487099
CGGCCAT37100.053.7448239
GCCATAC37550.052.91060611
CCGAACT12550.052.652667
CCATACC38950.051.10286312
GGGTCTA30550.051.038542
CGCGGTG87100.050.6564910
TGGCGCG87350.050.58913815
CATACCA39800.050.01147513
GCCGGTT13200.049.9149323
GGCCATA40400.049.44016610
CCTGGTT56800.049.1065270
CCGGTTC13750.047.918984
GAACGCG42300.047.2172124
CTGAACG42150.047.20157622
Produced by FastQC (version 0.11.5)