FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3709238
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG206460.5566102795237189No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC99750.268923158880611No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC82200.22160885874672911No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG76490.2062148613812325No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG61450.1656674497565268No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG58800.1585231252348865No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT53260.1435874430273819No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT46170.12447300496759711No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44810.12080648370366097No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA44240.1192697799386289No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT38590.10403754086418827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT17400.039.56337770
CGCGGCA62800.036.35547332
GCGGCAC65200.034.97215333
CGGCACC66600.034.50511634
CCGTCGC63750.034.3126845
CCCGTCG64300.034.22187444
TCGCCGG64500.034.01868448
GCACCCC71250.032.42860436
CGTACGT1600.032.3702719
CCCCCGA71650.032.09468519
AGGACCG6050.032.02853470
ACCCGCG73050.031.37932625
CACCCCC73750.031.10805737
CCCCGAC75500.030.27963620
GGCACCC76900.029.89524835
ATGGCCG18050.029.4853469
GTTCCGT4200.028.9972131
CGTCGCC76750.028.56248946
CCGCGCG80250.028.4943927
AGTCCGC80950.028.3460487