FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9775
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN570.5831202046035806No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG430.43989769820971864No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.42966751918158563No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG230.2352941176470588No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC210.21483375959079282No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG160.1636828644501279No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA140.14322250639386191No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT140.14322250639386191No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT130.1329923273657289No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG120.1227621483375959No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG110.11253196930946291No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT100.10230179028132991No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC100.10230179028132991No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC100.10230179028132991No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG100.10230179028132991No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA100.10230179028132991No Hit
AGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAG100.10230179028132991No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGG206.295315E-572.54143562
GGCGCGG206.651605E-571.74863461
CCCGTCG150.002176312769.8404244
CCCCGTC150.002176312769.8404243
TCGCCGG207.612919E-569.8404248
CCCCCGT150.002176312769.8404242
GCACCCC208.027065E-569.1052636
GCGGCAC208.240757E-568.74345433
GCGCGGC208.240757E-568.74345431
CGCGGCA208.240757E-568.74345432
CGGCACC208.240757E-568.74345434
CGCGCGG208.240757E-568.74345430
ACCCGCG208.458953E-568.38541425
CGACCCG208.458953E-568.38541423
ACAGTCC208.6817236E-568.031085
CAGTCCG208.6817236E-568.031086
GTCCGCC208.6817236E-568.031088
CCCCGAC208.6817236E-568.0310820
TCCGCCC208.6817236E-568.031089
AGTCCGC208.6817236E-568.031087
Produced by FastQC (version 0.11.5)