FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10009
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN570.5694874612848436No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA170.16984713757618145No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC160.15985612948346486No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG160.15985612948346486No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT140.13987411329803176No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC140.13987411329803176No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC120.11989209711259866No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCTG150.00179792173.2756867
CGGGCCT150.00183711172.8817266
GGTCGGG150.00183711172.8817263
GGGTCGG150.001876934472.4919862
AGCTAAG150.002042699470.9738253
CTAAGCA150.002042699470.9738255
GCTAAGC150.002042699470.9738254
GAAGCTA150.002085792470.6041751
CGGAAGC150.002085792470.6041749
AAGCTAA150.002085792470.6041752
TCTCGGA150.002219144569.5179546
ATCTCGT150.00226497669.1632735
AGCTACT150.00226497669.1632735
GTCTGAT150.00226497669.1632740
GCTACTC150.00226497669.1632736
CTCGGGA150.00226497669.1632740
CTACTCG150.00226497669.1632737
TCGGGAG150.00226497669.1632741
ACTCGGG208.006562E-569.1632639
TACTCGG208.006562E-569.1632638
Produced by FastQC (version 0.11.5)