FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005319199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005319199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3773531
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA114090.3023428189671689No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC113190.29995778489695724No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC104140.2759749423020508No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG81060.2148120685903998No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT47800.1266718095067988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG19000.058.486084
TACGGCC19750.056.089817
TCTACGG19950.055.8745545
CTACGGC20100.055.1131256
GCGCGGT60700.052.4116639
TGGCGCG63750.049.5756815
CGCGGTG64500.049.4848510
CCGAACT10050.047.7213267
ACGGCCA23750.047.2261438
CGGCCAT24050.046.349169
GCCATAC24000.046.0044611
CGGTTCA10900.045.4161875
GCCGGTT10750.045.403443
GAACGCG24450.044.92466424
CCATACC24650.044.65686412
CGCGTGC72050.044.06561318
CGAACTT11300.043.96146868
ATCTCGT25000.043.8664235
CCCGATC24950.043.7884631
GGGTCTA21550.043.3705142
Produced by FastQC (version 0.11.5)